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anti human stro 1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti human stro 1
    Anti Human Stro 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+stro+1+antibody/STRO-1+Antibody/us12480095-1103-30-32
    Average 95 stars, based on 212 article reviews
    anti human stro 1 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Bone Marrow Mesenchymal Stromal Cell Osteogenesis is driven by Paracrine signals from Regulatory T Cell.
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde (Sigma-Aldrich) for 10 min, permeabilized (Invitrogen) for 30 min, and blocked with 3% BSA and 10% Normal Goat Serum (Thermo Fisher Scientific) in DPBS for 1 h. Cells were then incubated overnight at 4 °C with anti-STRO-1 antibody (1:150) (Santa Cruz Biotechnology, USA) in 3% BSA/DPBS, and followed by Alexa FluorTM 488 secondary antibody (1:200) (Thermo Fisher Scientific) in DPBS for 1 h. Nuclei and cytoskeleton was stained with DAPI (1:1000) (Thermo Fisher Scientific) and Phalloidin Atto 565 (1:500) (Sigma-Aldrich) in DPBS for 1 h. Images were taken by Dragonfly 505 confocal microscope (Andor Technologies Inc, Northern Ireland). .. Treg were isolated from six healthy donors and expanded for 13 days with TexMACS medium (Miltenyi Biotec) supplemented with 5% AB serum and 500 U/mL IL-2.

    Article Title: Bone Marrow Mesenchymal Stromal Cell Osteogenesis is driven by Paracrine signals from Regulatory T Cell
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde (Sigma-Aldrich) for 10 min, permeabilized (Invitrogen) for 30 min, and blocked with 3% BSA and 10% Normal Goat Serum (Thermo Fisher Scientific) in DPBS for 1 h. Cells were then incubated overnight at 4 °C with anti-STRO-1 antibody (1:150) (Santa Cruz Biotechnology, USA) in 3% BSA/DPBS, and followed by Alexa FluorTM 488 secondary antibody (1:200) (Thermo Fisher Scientific) in DPBS for 1 h. Nuclei and cytoskeleton was stained with DAPI (1:1000) (Thermo Fisher Scientific) and Phalloidin Atto 565 (1:500) (Sigma-Aldrich) in DPBS for 1 h. Images were taken by Dragonfly 505 confocal microscope (Andor Technologies Inc, Northern Ireland). .. Treg were isolated from six healthy donors and expanded for 13 days with TexMACS medium (Miltenyi Biotec) supplemented with 5% AB serum and 500 U/mL IL-2.

    Article Title: Expanding upon the Human Myometrial Stem Cell Hypothesis and the Role of Race, Hormones, Age, and Parity in a Profibroid Environment
    Article Snippet: .. Samples were incubated with anti–STRO-1 antibody (sc-47733, Santa Cruz Biotechnology, Dallas, TX) overnight at 4°C. .. Samples were then washed, and bound mouse anti–Stro-1 primary antibodies were detected using anti-mouse secondary antibody (Vector Laboratories) during a 30-minute incubation at room temperature, then washed.

    Staining:

    Article Title: Bone Marrow Mesenchymal Stromal Cell Osteogenesis is driven by Paracrine signals from Regulatory T Cell.
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde (Sigma-Aldrich) for 10 min, permeabilized (Invitrogen) for 30 min, and blocked with 3% BSA and 10% Normal Goat Serum (Thermo Fisher Scientific) in DPBS for 1 h. Cells were then incubated overnight at 4 °C with anti-STRO-1 antibody (1:150) (Santa Cruz Biotechnology, USA) in 3% BSA/DPBS, and followed by Alexa FluorTM 488 secondary antibody (1:200) (Thermo Fisher Scientific) in DPBS for 1 h. Nuclei and cytoskeleton was stained with DAPI (1:1000) (Thermo Fisher Scientific) and Phalloidin Atto 565 (1:500) (Sigma-Aldrich) in DPBS for 1 h. Images were taken by Dragonfly 505 confocal microscope (Andor Technologies Inc, Northern Ireland). .. Treg were isolated from six healthy donors and expanded for 13 days with TexMACS medium (Miltenyi Biotec) supplemented with 5% AB serum and 500 U/mL IL-2.

    Article Title: Bone Marrow Mesenchymal Stromal Cell Osteogenesis is driven by Paracrine signals from Regulatory T Cell
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde (Sigma-Aldrich) for 10 min, permeabilized (Invitrogen) for 30 min, and blocked with 3% BSA and 10% Normal Goat Serum (Thermo Fisher Scientific) in DPBS for 1 h. Cells were then incubated overnight at 4 °C with anti-STRO-1 antibody (1:150) (Santa Cruz Biotechnology, USA) in 3% BSA/DPBS, and followed by Alexa FluorTM 488 secondary antibody (1:200) (Thermo Fisher Scientific) in DPBS for 1 h. Nuclei and cytoskeleton was stained with DAPI (1:1000) (Thermo Fisher Scientific) and Phalloidin Atto 565 (1:500) (Sigma-Aldrich) in DPBS for 1 h. Images were taken by Dragonfly 505 confocal microscope (Andor Technologies Inc, Northern Ireland). .. Treg were isolated from six healthy donors and expanded for 13 days with TexMACS medium (Miltenyi Biotec) supplemented with 5% AB serum and 500 U/mL IL-2.

    Microscopy:

    Article Title: Bone Marrow Mesenchymal Stromal Cell Osteogenesis is driven by Paracrine signals from Regulatory T Cell.
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde (Sigma-Aldrich) for 10 min, permeabilized (Invitrogen) for 30 min, and blocked with 3% BSA and 10% Normal Goat Serum (Thermo Fisher Scientific) in DPBS for 1 h. Cells were then incubated overnight at 4 °C with anti-STRO-1 antibody (1:150) (Santa Cruz Biotechnology, USA) in 3% BSA/DPBS, and followed by Alexa FluorTM 488 secondary antibody (1:200) (Thermo Fisher Scientific) in DPBS for 1 h. Nuclei and cytoskeleton was stained with DAPI (1:1000) (Thermo Fisher Scientific) and Phalloidin Atto 565 (1:500) (Sigma-Aldrich) in DPBS for 1 h. Images were taken by Dragonfly 505 confocal microscope (Andor Technologies Inc, Northern Ireland). .. Treg were isolated from six healthy donors and expanded for 13 days with TexMACS medium (Miltenyi Biotec) supplemented with 5% AB serum and 500 U/mL IL-2.

    Article Title: Bone Marrow Mesenchymal Stromal Cell Osteogenesis is driven by Paracrine signals from Regulatory T Cell
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde (Sigma-Aldrich) for 10 min, permeabilized (Invitrogen) for 30 min, and blocked with 3% BSA and 10% Normal Goat Serum (Thermo Fisher Scientific) in DPBS for 1 h. Cells were then incubated overnight at 4 °C with anti-STRO-1 antibody (1:150) (Santa Cruz Biotechnology, USA) in 3% BSA/DPBS, and followed by Alexa FluorTM 488 secondary antibody (1:200) (Thermo Fisher Scientific) in DPBS for 1 h. Nuclei and cytoskeleton was stained with DAPI (1:1000) (Thermo Fisher Scientific) and Phalloidin Atto 565 (1:500) (Sigma-Aldrich) in DPBS for 1 h. Images were taken by Dragonfly 505 confocal microscope (Andor Technologies Inc, Northern Ireland). .. Treg were isolated from six healthy donors and expanded for 13 days with TexMACS medium (Miltenyi Biotec) supplemented with 5% AB serum and 500 U/mL IL-2.

    Northern Blot:

    Article Title: Bone Marrow Mesenchymal Stromal Cell Osteogenesis is driven by Paracrine signals from Regulatory T Cell.
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde (Sigma-Aldrich) for 10 min, permeabilized (Invitrogen) for 30 min, and blocked with 3% BSA and 10% Normal Goat Serum (Thermo Fisher Scientific) in DPBS for 1 h. Cells were then incubated overnight at 4 °C with anti-STRO-1 antibody (1:150) (Santa Cruz Biotechnology, USA) in 3% BSA/DPBS, and followed by Alexa FluorTM 488 secondary antibody (1:200) (Thermo Fisher Scientific) in DPBS for 1 h. Nuclei and cytoskeleton was stained with DAPI (1:1000) (Thermo Fisher Scientific) and Phalloidin Atto 565 (1:500) (Sigma-Aldrich) in DPBS for 1 h. Images were taken by Dragonfly 505 confocal microscope (Andor Technologies Inc, Northern Ireland). .. Treg were isolated from six healthy donors and expanded for 13 days with TexMACS medium (Miltenyi Biotec) supplemented with 5% AB serum and 500 U/mL IL-2.

    Article Title: Bone Marrow Mesenchymal Stromal Cell Osteogenesis is driven by Paracrine signals from Regulatory T Cell
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde (Sigma-Aldrich) for 10 min, permeabilized (Invitrogen) for 30 min, and blocked with 3% BSA and 10% Normal Goat Serum (Thermo Fisher Scientific) in DPBS for 1 h. Cells were then incubated overnight at 4 °C with anti-STRO-1 antibody (1:150) (Santa Cruz Biotechnology, USA) in 3% BSA/DPBS, and followed by Alexa FluorTM 488 secondary antibody (1:200) (Thermo Fisher Scientific) in DPBS for 1 h. Nuclei and cytoskeleton was stained with DAPI (1:1000) (Thermo Fisher Scientific) and Phalloidin Atto 565 (1:500) (Sigma-Aldrich) in DPBS for 1 h. Images were taken by Dragonfly 505 confocal microscope (Andor Technologies Inc, Northern Ireland). .. Treg were isolated from six healthy donors and expanded for 13 days with TexMACS medium (Miltenyi Biotec) supplemented with 5% AB serum and 500 U/mL IL-2.

    Flow Cytometry:

    Article Title: Patient-Derived Human Induced Pluripotent Stem Cells From Gingival Fibroblasts Composited With Defined Nanohydroxyapatite/Chitosan/Gelatin Porous Scaffolds as Potential Bone Graft Substitutes
    Article Snippet: .. The differentiated cells were then collected with 0.05% trypsin/EDTA (Invitrogen), sorted by flow cytometry with anti-stro-1 antibody (Santa Cruz Biotechnology Inc., Santa Cruz, CA, http://www.scbt.com ). ..



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    Santa Cruz Biotechnology anti human stro 1
    Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and <t>stro-1,</t> and negative to cytokeratin-3 of hDPSCs
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    Santa Cruz Biotechnology anti stro 1 antibody
    Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and <t>stro-1,</t> and negative to cytokeratin-3 of hDPSCs
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    Image Search Results


    Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and stro-1, and negative to cytokeratin-3 of hDPSCs

    Journal: BMC Oral Health

    Article Title: Salvianolic acid B functionalized injectable GelMA hydrogel for pulpitis in a vital pulp therapy: a dual anti-inflammatory property and enhanced reparative dentinogenesis activity material

    doi: 10.1186/s12903-026-07880-z

    Figure Lengend Snippet: Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and stro-1, and negative to cytokeratin-3 of hDPSCs

    Article Snippet: Primary antibodies included rabbit anti-human nestin (Affinity Biosciences, China), mouse anti-human stro−1 (R&D systems, USA) and mouse anti-human cytokeratin (Abcam, USA).

    Techniques: Light Microscopy, Staining, Immunofluorescence